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I want to know if the sentence below makes any sense?
Of this method of characterizing the size of active sites have been attempted with varying success
Context is important. Please provide enough for us to be able to deal effectively with your question.
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If you remove the first word, change "have" and perhaps add a couple of words towards the end of the sentence, it stands a chance of being intelligible. At the moment, it means nothing.
Remember - correct capitalisation, punctuation and spacing make posts much easier to read.