No, sorry. Partly, that's because when you use a negative, it starts to get a bit complicated: Is "I don't like it the worst" a sort of double negative? (Slavic languages allow double negatives; formal English interprets double negatives as positives.)
Use a positive verb, and use more/most or less/least as appropriate:
Which book did you like less?
Which book did you dislike/hate more?
Which subject do you like the least?
Which subject do you dislike/hate the most?
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