Due to the increased survival rate after treatment for cancer patients, especially children that reach reproductive age, treatment of infertility after cancer treatment in this population will be doubled. The most common method of fertility preservation in male cancer patients, their sperm for in vitro fertilization using frozen semen and after cancer treatment. Due to lack of active spermatogenesis in children, Preservation of testicular tissue or germ cells to be frozen before cancer treatment is considered an appropriate treatment. Recently, many researches have been done to optimize of Cryopreservation media