No. And "play" should be "plays".
Interested in Language
Metaphorically, can I say "God is the one who shuffles cards and you are the one who play (the) cards".
Does the sentence sound natural with or without "the"?
It would be more natural like this: "God is the one who shuffles the cards and you are the one who plays them".
God shuffles the cards, you play the hand you're dealt.
But note that this requires either a conjunction ("... the cards, and you play...) or some other form of punctuation besides a comma.God shuffles the cards, you play the hand you're dealt.
I'm not a teacher, but I write for a living. Please don't ask me about 2nd conditionals, but I'm a safe bet for what reads well in (American) English.