Hello, This is Pranav. I will be grateful if anyone could check my LOM. Thank you in advance.

‘It is not just a statement of purpose – it is a quest to realize my passion for

Alzheimer’s research’

I decided to pursue a PhD in neurodegenerative disease, specifically Alzheimer’s disease, during the final year of studies for my bachelor’s degree. To that end, I pursued a master's with the Department of Pharmacology, my thesis on Alzheimer’s disease; and my goal now is to seek PhD on Alzheimer’s disease at the molecular level, also its pharmacological treatment and ultimately to make a contribution in combating the disease.

With the perpetual interest towards working on AD, I pursued Masters in the department of Pharmacology. I carried out my thesis work on the behavioral aspects of AD using Zebra fish. I was always interested to learn a new animal model and for that reason I decided to work on zebra fish model. I did my project in our college which gave a great insight into the research methodology beginning from the literature survey to the write-up of results. I worked on the assessment of memory enhancing activity using Ocimum sanctum leaves aqueous extract using two behavioral tests, inhibitory avoidance test (IAT) and color biased appetite conditioning T-maze. I noticed that the test drug treated animals showed hindrance to enter the dark chamber (Where shock treatment was given during training session) in the test session in IAT and in T-maze, the animals entered food containing green chamber and also had less total number of entries which indicated the enhancement of memory in the animals treated with the test drug when compared to control group.

During under graduation, as a part of course curriculum I learnt molecular biology techniques like Gel electrophoresis, ELISA, bacterial culture, western blotting. During my post-graduation, I handled different laboratory animals like rodents (rats and mice), rabbit, guinea pig and also zebra fish. I learnt different routes of administration in rodents like Intravenous (I.V), Intraperitoneal (I.P), Sub-cutaneous (S.C), oral (P.O), Intracerebroventricular (I.C.V) and in zebrafish like Oral and Intraperitoneal (I.P). I also learnt breeding techniques and isolation of brain in zebra fish. I also handled many equipments like Kymograph, Plethysmograph, Pole Climbing apparatus, Actophotometer, Electro-convulsiometer, Centrifuge, Incubator and mazes (Plus, T and water). With the sound knowledge on behavior analysis, I could develop a behavioral model by the support bestowed by my faculty and was able to employ it in my post graduation thesis work. We were exhilarated after it worked well with the experiment. I feel proud to say that this work had also been published in a journal with the title “Design and development of a novel color bias avoided and appetite conditioning T maze model for evaluating the memory enhancing drugs in zebra fish”.

With a great enthusiasm to meet the eminent professors of my field, I also energetically participated in various conference and presented posters during under-graduation and post-graduation studies. The most outstanding amongst all was Indo-American Pharmaceutical Regulatory Symposium organized by North Eastern University, Boston, USA at one of the premiere universities, Jawaharlal Nehru Technological University where I presented poster on ‘Neuronal calcium signaling in Alzheimer’s disease’ which offered me worthful opportunity to meet great professors. I actively participated in many extracurricular activities like singing, dance during bachelor’s and master’s studies for which I also received a prize.

The pathetic life of Alzheimer’s patient (My Grand Mother) and also the stressful life of the caregivers (Grand Father and My Mother) ignited my interest towards Alzheimer’s disease. With this motivation, I decided to further my career in the field of research on the same. My research interest is mainly on studying the molecular mechanisms of Alzheimer’s disease (AD). To be specific, my major interest is to study the formation of Amyloid β plaques, and Neurofibrillary tangles which are the major pathological hallmarks of the disease. In addition, I am also interested in carrying out studies on the other molecular mechanisms and potential treatment of Alzheimer’s disease.

I always intended to be a part of organization which provides state-of-the-art research facilities, great research environment, boundless support, friendly academic community. Your university meets all the criteria. I studied your article ‘Decreased Activation along the dorsal visual pathway after a 3-month treatment with Galantamine in Mild Alzheimer Disease’. It was really an interesting work which indicates that the visual perception is altered in AD patients. I found that you are mainly involved in developing non-invasive markers for AD. I am enthusiastic to work under your guidance in your laboratory where I get chance to directly interact with AD patients. My long term goals are to secure PhD position in Alzheimer's research then seek a post doctoral position and further carry out my career in the research field as a research scientist. I also love to disseminate my knowledge to the students. My desire to pursue PhD, specifically on Alzheimer’s disease at your university will satiate my thirst for subject & provide an opportunity to scale that ladder of success in the field of research.

Thank you for the opportunity to express my views. Kindly contact me at any time as I am looking for good news. Thank you a lot for your valuable time and kind consideration.

Vemula Pranav Kumar.