Yes, you can. But "I" needs to be capitalized.
Student or Learner
I ask her again do i need to activate the card or not.
I ask her whether should I need to activate the card.
How to use the "or not", can I say as the sentence above?
Are you using the present tense because you haven't met the past? The verb 'ask' sounds a bit odd when used in the present tense - in most cases. (The main exception is in rhetoric: 'I ask you, is that any way to treat a colleague' - this is not really a question; the speaker expects everyone in the audience to agree that the answer is "No".)
In my examples, I use the simple past - just because it feels more likely.
I asked her again 'Do I need to activate the card or not?'
[The 'or not' in unnecessary, but can be used to express emphasis.]
I asked her whether I needed to activate the card.