In this case, no. "Have to" is an alternative way of writing "must", and "must" is a modal verb. Modal verbs express posibility or compulsion, and you cannot (if you think about it logically) order people to be compelled to do something. You just order them to do something, and there's the compulsion right there, without needing any extra modality.
In short: you cannot use a modal verb or any of its alternatives to form an imperative.
There are at least three ways to do this:
1. Use a normal affirmative statement with the modal verb and a subject: "You must use gloves" or "You must wear gloves."
2. Use a passive construction with the modal verb: "Gloves must be used" or "Gloves must be worn".
3. Use an imperative without the modal: "Use gloves" or "Wear gloves."
In most formal situations -- instruction manuals, warning signs etc -- the passive construction is best.