Yes.
Hello.
Can I omit "at" in my sentence?
"See you on Saturday, four o'clock."
Yes.
You can even omit "on" in casual speech. These all work:
See you on Saturday at four o'clock.
See you on Saturday at four.
See you Saturday at four o'clock.
See you Saturday at four.
See you Saturday - four o'clock.
Note the dash in the final example. There would be a clear break between "Saturday" and "four o'clock" when spoken aloud.
Remember - if you don't use correct capitalisation, punctuation and spacing, anything you write will be incorrect.